Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCC1

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
diagnosis
rhabdomyosarcoma
treatment
7 days treatment with 250nM cis conformation (inactive) PROTAC compound (University of Dundee, Institute of Chemical and Structural Biology available at Boehringer Ingelheim (opnMe))
genotype
wild type
antibody
anti-SMARCC1, Abcam, ab172638

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After reverse crosslinking, ChIP DNA was purified using MinElute PCR Purification Kit (Qiagen). ChIPseq Library

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
28202644
Reads aligned (%)
80.6
Duplicates removed (%)
64.2
Number of peaks
1755 (qval < 1E-05)

hg19

Number of total reads
28202644
Reads aligned (%)
75.2
Duplicates removed (%)
63.3
Number of peaks
1828 (qval < 1E-05)

Base call quality data from DBCLS SRA